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hcov 229e spike  (Sino Biological)
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Identification of broadly neutralizing antibodies against human betacoronaviruses The area under the curve (AUC) from titration of mAb binding to spike proteins from human betacoronaviruses SARS-CoV-2 Wuhan-Hu-1 (CoV-2), SARS-CoV (CoV-1), MERS-CoV, HCoV-HKU1, and HCoV-OC43, as well as alphacoronaviruses HCoV-NL63 and HCoV-229E, is shown on the panel of the left. Influenza H1 hemagglutinin (HA) was included as a control antigen and L9 IgG1 (malaria specific; <xref ref-type=Wang et al., 2020 ) was included as a negative control mAb for binding experiments. AUC values for each antigen are shown after subtraction with values for the negative control antigen CD4. The antibody titers at 50% neutralization (NT 50 ) against SARS-CoV-2 Wuhan-Hu-1, SARS-CoV, MERS-CoV, HCoV-NL63 envelope-pseudotyped virus, as well as authentic HCoV-OC43, are shown on the right. Neutralizing mAbs are ranked by their breadth of neutralization and the geometric mean of their NT 50 values. Cells highlighted in blue denote mAbs that did not show neutralizing potency at the highest concentration tested (100 μg/mL). Negative control mAbs for neutralization are DEN3 (dengue-specific; Rogers et al., 2020 ) for SARS-CoV-2, SARS-CoV, MERS-CoV and HCoV-NL63, and CV503 (SARS-CoV-2 RBD-specific; Cho et al., 2021 ) for HCoV-OC43. NT 50 values were calculated using the dose-response-inhibition model with 5-parameter Hill slope equation in GraphPad Prism version 9.3.1. " width="100%" height="100%">

Journal: Cell Host & Microbe

Article Title: Rare, convergent antibodies targeting the stem helix broadly neutralize diverse betacoronaviruses

doi: 10.1016/j.chom.2022.10.010

Figure Lengend Snippet: Identification of broadly neutralizing antibodies against human betacoronaviruses The area under the curve (AUC) from titration of mAb binding to spike proteins from human betacoronaviruses SARS-CoV-2 Wuhan-Hu-1 (CoV-2), SARS-CoV (CoV-1), MERS-CoV, HCoV-HKU1, and HCoV-OC43, as well as alphacoronaviruses HCoV-NL63 and HCoV-229E, is shown on the panel of the left. Influenza H1 hemagglutinin (HA) was included as a control antigen and L9 IgG1 (malaria specific; Wang et al., 2020 ) was included as a negative control mAb for binding experiments. AUC values for each antigen are shown after subtraction with values for the negative control antigen CD4. The antibody titers at 50% neutralization (NT 50 ) against SARS-CoV-2 Wuhan-Hu-1, SARS-CoV, MERS-CoV, HCoV-NL63 envelope-pseudotyped virus, as well as authentic HCoV-OC43, are shown on the right. Neutralizing mAbs are ranked by their breadth of neutralization and the geometric mean of their NT 50 values. Cells highlighted in blue denote mAbs that did not show neutralizing potency at the highest concentration tested (100 μg/mL). Negative control mAbs for neutralization are DEN3 (dengue-specific; Rogers et al., 2020 ) for SARS-CoV-2, SARS-CoV, MERS-CoV and HCoV-NL63, and CV503 (SARS-CoV-2 RBD-specific; Cho et al., 2021 ) for HCoV-OC43. NT 50 values were calculated using the dose-response-inhibition model with 5-parameter Hill slope equation in GraphPad Prism version 9.3.1.

Article Snippet: HCoV-229E spike , Sino Biological , Cat. # 40605-V08B-B.

Techniques: Titration, Binding Assay, Negative Control, Neutralization, Concentration Assay, Inhibition

Journal: Cell Host & Microbe

Article Title: Rare, convergent antibodies targeting the stem helix broadly neutralize diverse betacoronaviruses

doi: 10.1016/j.chom.2022.10.010

Figure Lengend Snippet:

Article Snippet: HCoV-229E spike , Sino Biological , Cat. # 40605-V08B-B.

Techniques: Recombinant, Transfection, Lysis, Cell Culture, Luciferase, Expressing, Irradiation, Amplification, Plasmid Preparation, Software, Fluorescence, Microscopy, Imaging